Biochemistry Laboratory

Laboratory Homepage: https://biochemistry.chem.uoa.gr

 

  Faculty Members

Emmanouilidou Evangelia (Assistant Professor, Lab Director)

Stratikos Efstratios (Associate Professor)

 

Retired Faculty Members

Demopoulos Constantinos (Professor)
Galanopoulou Dia (Professor)
Mavri-Vavagianni Maria (Associate Professor)
Siafaka-Kapadai Athanasia (Professor)

 

Administrative Staff

 

Technical Staff

 

Deceased Faculty Members

 

 

RESEARCH FIELDS

  • Lipid chemistry and biochemistry.Isolation, identification and determination of lipid molecules in food of plant and animal origin, plant and animal tissues, body fluids, plant and animal cell cultures, unicellular eukaryote cultures.
  • Studies on the allergenic activity of food.
  • Cardiovascular diseases and Mediterranean diet: Studies on the mechanism of atherogenesis, biochemical approach to the protective role of Mediterranean diet.
  • Effect of dietary and endogenous factors on the differentiation of adipocytes. Studies of the mechanisms of signal transduction in adipocytes and preadipocytes.
  • Platelet-Activating Factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine, PAF): Isolation and determination from natural sources, metabolism, mode of action, implication in diseases and its pathophysiological role, inhibitors, compounds with PAF-like activity.
  • Study of the endocannabinoid system in eukaryotic cells: receptors, enzymes and endocannabinoids. Synthesis and study of endocannabinoid derivatives.
  • Fatty acids as signaling molecules: oleic acid, arachidonic acid and derivatives, trans-fatty acids and derivatives. Oxidative stress and fatty acids.
  • Mechanism of action of valproic acid, synthesis and study of derivatives.
  • Biochemistry and analysis of phosphoinositides. Participation of phosphoinositides to cell signaling and regulation of intracellular membrane traffic.
  • Cell signaling via lipids in plants
  • Teaching Biochemistry in Secondary education

INSTRUMENTATION

  • High Performance Liquid Chromatography (HPLC)
  • Gas Chromatography (GC)
  • UV spectrophotometers, simple photometers
  • Centrifuges
  • pHmeters
  • Water baths
  • Autoclaves
  • Ultrasonic bath – Ultrasonic devices
  • Rotary Evaporators
  • Fractioncollector
  • Freeze dryer
  • Aggregometer
  • Incubators, CO2 incubator
  • Constant temperaturechamber
  • Laminar (vertical/horizontal) flow hoods
  • Electrophoresis apparatuses, protein transfer device
  • PCRdevice
  • Optical microscopes and inverted phase microscope
  • Distilled water and Millipore water purification systems
  • Ice machine
  • Liquid ScintillationCounter
  • Radioisotope use license
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